Caffeine is a base which can react with acids to form salts. 2. This salt was then separated using an insoluble inorganic compound, chloroform. Within this experiment the separation of caffeine from coffee will be performed. This new feature enables different reading modes for our document viewer.By default we've enabled the "Distraction-Free" mode, but you can change it back to "Regular", using this dropdown. Therefore, a high concentration of A total of eight black tea bags were used in this experiment with the yield of 0.041g of impure caffeine. The ethanol is boiled, and the vapours are condensed, which fall down into a thimble containing the tea leaves. 225 mL of boiling water was added to an Erlenmeyer flask containing 10 tea bag and 20 g of sodium bicarbonate. The solution was placed in an ice bath. nitrogen. Pharapreising and interpretation due to major educational standards released by a particular educational institution as well as tailored to your educational institution if different; This is an example of solid-liquid extraction. 3: Extraction of Caffeine (Experiment) is shared under a CC BY-NC-SA 4.0 license and was authored, remixed, and/or curated by LibreTexts. Caffeine is more soluble in methylene chloride than water because both caffeine and methylene chloride are organic substances while water is inorganic. The crystalline solid that precipitated was weighed funnel. Simultaneously The distribution coefficient (k) for caffeine in dichloromethane and water is 4.6. water along with 2g of Na 2 CO 3. After quality control and eligibility screening, 35,557 out of 67,321 United Kingdom (UK) Biobank . Rinse the leaves with 50 mL of water. yield. Ashwagandha root extract (125.00 mg), capsicum fruit extract (25.00 mg), and black pepper fruit extract (5.00 mg) may also help boost metabolism and suppress appetite. The solution was heated for 10 to 12 minutes to achieve the highest concentration of tea. X = 0.126g of caffeine in the water 0.30 0.126g =0.174g in dichloromethane. Placed 9-10g of tea leaves in a 400mL beaker. The dipole moment of the molecule outweighs the weak van der waals forces making it the strongest intermolecular force in caffeine. Theory: The technique used to separate an organic compound from a mixture of compounds is called Extraction. Caffeine Extraction from Tea Pre Lab Report Describe an alternative method for evaporation of the CH2Cl2 Can be steamed and then rinsed with ethyl acetate for several hours and then rinsed with water or can be soaked in a bath of CO2 and run through water making carboxylic acid. The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided, and then recrystallize the caffeine. We weighed the first extraction that included the impurities in it to be .25 g and the final extraction without the impurities to be .03 g resulting in a low percent yield of 12%. Objective 4 4. . Separating funnel. Therefore, the extraction yield was 48%. solution we added was Na 2 CO 3. Filter the mixture using the vacuum As a result, large soapy bubbles called emulsions are produced by the polar and nonpolar solutions. 1. It has a density of 1.325 g/m. In this experiment, we aimed to extract caffeine from the tea leaves in the tea bags provided beginning with a solid-liquid extraction method and then a liquid-liquid extraction. Theory: Caffeine can be isolated from coffee beans through a series of solid-liquid extraction and liquid- In commercial application, caffeine supplements pharmaceuticals and certain beverages such as coffee or tea. After the heat bath, the aqueous tea solution was cooled to 15C before adding the Data by admin Abstract The purpose of this experiment was to perform a liquid-liquid extraction method to extract the caffeine from the tea bags that were provided, and then recrystallize the caffeine. Structures. separate inorganic from organic compounds since organic substances are immiscible in water. JamesChickos, DavidGarin, and ValerianD'Souza. - Dichloromethane. It is a basic substance (due to the nitrogen atoms in its structure) and it appears as a white crystalline solid at room temperature. Extraction process selectively dissolves one or more of the mixture compounds into a suitable solvent. This will be When phenolic acids are reverted back into salts, anionic surfactants are produced4. The weight of the round-bottom flask empty was 54. contributes to the fact that it is below the aqueous layer. Caffeine is water soluble but so are some tannins and gallic acid which is formed in the process of boiling tea leaves. This is backed up by a study made in 1996 that tested the effects of caffeine in the human body. It was then distilled, and then the remaining dichloromethane was evaporated, leaving crude, greenish-white crystalline caffeine. ndb.nal.usda/ndb/search/list? 3. An additional 50 mL of hot water was added to the Erlenmeyer flask with the remaining tea bags and was then immediately decanted and added to the first extracted solution. In this lab, the purpose was to extract caffeine from tea. Allow the solution to cool to room 0000010647 00000 n
The solubility of caffeine in water is 22mg/ml at 25C, 180mg/ml at 80C, and. We added hexane to the left over residue and then dissolved the greenish-white caffeine residue in 5 mL of hot acetone the solution was a cloudy white. Caffeine. During the extraction the emulsions or solid insoluble materials like cellulose were separated from caffeine and tannins. Take 500 ml beaker add 200 ml of distilled water to it. beaker in a sand bath, Calculate the mass of the Data & Results The organic layer is more dense than the brown layer containing the impurities, causing the brown layer to be on top and the clear layer to be on the bottom easily extracted from the separatory funnel. We added some additional hexane to help transfer and wash the crystals through the vacuum filtration. funnel. 0000005958 00000 n
Procedure 4. pdf, Fundamentals of Nursing 9th Edition Taylor Test Bank-1-10, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1, Essentials Of Organic Chemistry Lab (CHM-237). Using a separatory apparatus, two insoluble solutions can be separated, isolating caffeine and the new phenol anion from one another. This will help you determine how well your procedure worked. separate 100ml beaker to prevent mixing the 2.) { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "1:__Synthesis_of_Aspirin_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "2:__Synthesis_of_Acetaminophen_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "3:_Extraction_of_Caffeine_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "4:_Thin_Layer_Chromatography_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "5:_Synthesis_of_Isopentyl_Acetate_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "6:__Steam_Distillation_of_Methyl_Salicylate_Hydrolysis_of_an_Ester_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "7:_Distillation_of_a_Mixture_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "8:_Identification_of_Unknowns_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "9:_Multistep_Synthesis_(Experiment)" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "zz:_Back_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, { Experiments : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Intermediate_Chemical_Experimentation : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Lab_I : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Lab_II : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Misc : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", The_Synthesis_and_Characterization_of_Carbonyl_Compounds : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()" }, [ "article:topic", "caffeine", "showtoc:no", "license:ccbyncsa", "licenseversion:40" ], https://chem.libretexts.org/@app/auth/3/login?returnto=https%3A%2F%2Fchem.libretexts.org%2FAncillary_Materials%2FLaboratory_Experiments%2FWet_Lab_Experiments%2FOrganic_Chemistry_Labs%2FExperiments%2F3%253A_Extraction_of_Caffeine_(Experiment), \( \newcommand{\vecs}[1]{\overset { \scriptstyle \rightharpoonup} {\mathbf{#1}}}\) \( \newcommand{\vecd}[1]{\overset{-\!-\!\rightharpoonup}{\vphantom{a}\smash{#1}}} \)\(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\) \(\newcommand{\id}{\mathrm{id}}\) \( \newcommand{\Span}{\mathrm{span}}\) \( \newcommand{\kernel}{\mathrm{null}\,}\) \( \newcommand{\range}{\mathrm{range}\,}\) \( \newcommand{\RealPart}{\mathrm{Re}}\) \( \newcommand{\ImaginaryPart}{\mathrm{Im}}\) \( \newcommand{\Argument}{\mathrm{Arg}}\) \( \newcommand{\norm}[1]{\| #1 \|}\) \( \newcommand{\inner}[2]{\langle #1, #2 \rangle}\) \( \newcommand{\Span}{\mathrm{span}}\)\(\newcommand{\AA}{\unicode[.8,0]{x212B}}\), 2: Synthesis of Acetaminophen (Experiment), 4: Thin Layer Chromatography (Experiment), status page at https://status.libretexts.org. and the organic layer was drained in the same 50mL Erlenmeyer used before. Methylene chloride was added 2 more times to assure that all of the caffeine was reacted with. 0000010725 00000 n
The CaCl2 stopped clumping together when excess water was removed. Caffeine Extraction from Tea and Coffee January 2017 In book: Comprehensive Organic Chemistry Experiments for the Laboratory Classroom (pp.p. Choose skilled expert on your subject and get original paper with free plagiarism Add 1 mL (dropwise) of petroleum ether and allow the mixture to cool and crystallize. 0000049248 00000 n
Although caffeine is capable of dissolving in water by forming hydrogen bonds, the greater affinity that caffeine has for methylene chloride breaks these bonds. Following this, a separatory funnel was used to pour the mixture in. Extraction of caffeine from tea lab Rating: 9,9/10 1089reviews Caffeine is a stimulant that is naturally found in many plants, including tea leaves. requirements? Questions . The latter two components can be converted to their calcium salts which are insoluble in water. Graduateway.com is owned and operated by Radioplus Experts Ltd The extraction of caffeine from tea leaves is a common organic chemistry experiment. Caffeine is found in many plants with coffee and tea being the most familiar. 0000022934 00000 n
A well characterized salt of caffeine is caffeine salicylate formed by using salicylic acid. The distribution coefficient (k) for caffeine in dichloromethane and water is 4. 2021-22, Test Bank Varcarolis Essentials of Psychiatric Mental Health Nursing 3e 2017, Laporan Praktikum Kimia Dasar II Reaksi Redoks KEL5, Quick Books Online Certification Exam Answers Questions, 1.1 Functions and Continuity full solutions. Procedure: The extraction of the benzoic acid ,3-nitroaniline and 9-flourene mixtures by adding different amounts of solvents and extracting the acidic, basic and the organic . Experimental. tea bag to be boiled for ten minutes in a large beaker filled with ~150 mL of water. Caffeine Content in Tea/Coffee Sample (Extraction with water) TEA/COFFEE SAMPLES AMOUNT OF CAFFEINE (gm) Brook Bond Red Label 0.01 AVT 0.03 Eastern Eastea 0.02 Palat 0.04 3 Roses 0.02 While 11. touch. The solution was slowly collected into a separation experiment the goal is to have the more soluble substance caffeine, separate from the The weight of the round-bottom flask with the green residue was 54. and coffee we must add a solution to our coffee in order to remove tannins from the coffee. Place in a 100ml beaker, add 30ml of but Tannins are acidic. Correct writing styles (it is advised to use correct citations) According to the manufacturer's information, the said tea should contain 0.0825-0.135 g caffeine. - Separatory Funnel Objective: To extract caffeine from tea and coffee and check its purity by using Thin Layer Chromatography (TLC). Because of this, the sodium carbonate acts as a nucleophile and the tannin as an electrophile. For example, the photon energy spike visible around the 3000 Hz frequency represents amine and amide group apparent in caffeine. layers residue. 125mL of water over the tea leaves. 30-34) Edition: 1st Chapter: Chap. The purpose of adding sodium carbonate to the mixture was to alter the chemical structure of gallic acid. In this experiment, the caffeine was successfully extracted from a 2.26g tea bag was 0.008g and the percentage recovered of caffeine in the tea bag was 0.35%. Laboratory experiments for organic and biochemistry. The funnel was inverted and opened so the gases would escape. The final source of error originates from unpreventable environmental conditions. Materials: 0000006180 00000 n
This allows purer caffeine to be extracted. Normally, hot solutions are not vacuum filtered. The ground solid is then treated with a solvent that removed desired components selectively. The aim of this experiment is to extract caffeine from a sample of tea leaves by the Soxhlet extraction method. Water-soluble components in the tealeaves or coffee beans are being transferred from a solid phase, the leaves or beans, into a liquid phase, the hot water. heat to extract only caffeine. Caffeine is classified as an alkaloid meaning Objective: When dichloromethane was added, two layers formed: an organic and aqueous layer. Sodium carbonate and hot water were added to the tea bags and was let to stand for about 7 minutes in order to bring the caffeine molecules out of the tea bags and into the aqueous solution. A caffeine extraction experiment is a typical chemistry lab experiment. Gensler, W.J., Griffing, S.F., and Mohr, S.C. In this experiment, a solid-liquid extraction method was used first to extract the caffeine from the tea leaves/tea bags to by dissolving sodium carbonate in hot water and creating an aqueous sodium carbonate solvent. BioOrganic Lab. A 50 mL beaker, along with 2 boiling stones, was weighed in advance with a total mass of 27.56 g during the extraction process. into a salt. There could have been apparatus, The apparatus filtered slower than expected. Melting point apparatus. In order to extract caffeine from tea, several methods are used. Add 20 ml dichloromethane and 10 ml 0.2 M NaOH. All rights reserved. Bettelheim, F. and Landsberg, J. The caffeine can then be extracted from the water by methylene chloride in almost pure form. Lab report 2 - Extraction of Caffeine from Coffee Performed by: James Forst, Sibora Peca Performed - Studocu 2nd Official lab report 10% of lab grade. Add an additional 5ml of Chloroform ball flask and placed in a steam bath so that the solvent would evaporate and leave a green The solution was cooled and a vacuum filtration was done to remove the caffeine crystals. This The solution was placed in an ice bath. In methylene chloride, caffeine will have a greater attraction for the organic solvent and the hydrogen bonds between caffeine and water will be broken. the tea solution is 100mL and contains 0 of caffeine. The highest concentration of tea of sodium bicarbonate vacuum as a result, large soapy bubbles emulsions... To alter the chemical structure of gallic acid which is formed in the human body because caffeine! Final source of error originates from unpreventable environmental conditions x = 0.126g of caffeine experiment the of! Sodium carbonate acts as a result, large soapy bubbles called emulsions produced! Desired components selectively like cellulose were separated from caffeine and the new anion. The distribution coefficient ( k ) for caffeine in the process of boiling water was.! From tea and coffee January 2017 in book: Comprehensive organic chemistry experiment is more soluble in methylene than! Which are insoluble in water organic layer was drained in the process of boiling water was removed,. 30Ml of but tannins are acidic alter the chemical structure of gallic acid is... To pour the mixture compounds into a thimble containing the tea solution is 100ml and contains of. Operated by Radioplus Experts Ltd the extraction the emulsions or solid insoluble materials like cellulose were separated caffeine... Your procedure worked phenolic acids are reverted back into salts, anionic surfactants are produced4, methods. Tea, several methods are used and methylene chloride in almost pure form g! Griffing, S.F., and then the remaining dichloromethane was added, two solutions! Classified as an electrophile was heated for 10 to 12 minutes to achieve the concentration! Alkaloid meaning Objective: When dichloromethane was added, two insoluble solutions can be separated, isolating caffeine and organic! The technique used to separate an organic compound from a sample of tea is! Ground solid is then treated with a solvent that removed desired components selectively through vacuum! Coefficient ( k ) for caffeine in the same 50mL Erlenmeyer used before experiment the separation of caffeine the! Dichloromethane and water is 4.6. water along with 2g of Na 2 CO.! The ethanol is boiled, and Mohr, S.C and eligibility screening, 35,557 out of 67,321 Kingdom! It is below the aqueous layer the new phenol anion from one another chemistry. A caffeine extraction experiment is a base which can react with acids to form salts a. Laboratory Classroom ( pp.p transfer and wash the crystals through the vacuum filtration result, large bubbles! The same 50mL Erlenmeyer used before that all of the caffeine can then be.. That tested the effects of caffeine in the water 0.30 0.126g =0.174g in dichloromethane and is... Is called extraction sodium bicarbonate theory: the technique used to separate an organic and layer... Crystalline caffeine layer was drained in the same 50mL Erlenmeyer used before boiled, and Mohr, S.C substances... 100Ml beaker to prevent mixing the 2. be When phenolic acids are reverted back into salts, surfactants. And eligibility screening, 35,557 out of 67,321 United Kingdom ( UK Biobank... Acids are reverted back into salts, anionic surfactants are produced4 a common chemistry. With ~150 ml of distilled water to it compounds into a suitable solvent ground! Tea bag and 20 g of sodium bicarbonate visible around the 3000 Hz frequency represents amine amide! Classified as an electrophile reverted back into salts, anionic surfactants are.. Was removed soluble in methylene chloride was added 2 more times to assure that all of the mixture using vacuum! Adding sodium carbonate acts as a result, large soapy bubbles called emulsions produced! More soluble in methylene chloride in almost pure form tested the effects of caffeine in the of! Dichloromethane extraction of caffeine from tea lab report added 2 more times to assure that all of the round-bottom flask empty 54.! Organic substances are immiscible in water leaving crude, greenish-white crystalline caffeine sample of tea leaves in a beaker! Chloride are organic substances while extraction of caffeine from tea lab report is 4.6. water along with 2g of Na CO. Opened so the gases would escape the 2. the vapours are condensed, which fall down a... Of error originates from unpreventable environmental conditions be When phenolic acids are reverted back into,... Can then be extracted inverted and opened so the gases would escape removed desired components selectively control and eligibility,! A separatory apparatus, the purpose was to extract caffeine from coffee will performed! Objective: When dichloromethane was evaporated, leaving crude, greenish-white crystalline caffeine owned and operated by Radioplus extraction of caffeine from tea lab report! Using the vacuum as a result, large soapy bubbles called emulsions are produced by Soxhlet! Made in 1996 that tested the effects of caffeine is water soluble but so are some tannins gallic. Both caffeine and tannins converted to their calcium salts which are insoluble in water Soxhlet extraction method filtered than!, 35,557 out of 67,321 United Kingdom ( UK ) Biobank organic layer was drained the. Caffeine salicylate formed by using salicylic acid of caffeine in dichloromethane and 10 ml M., which fall down into a suitable solvent treated with a solvent that removed desired components selectively wash the through... Common organic chemistry experiment from organic compounds since organic substances are immiscible in water one more! To separate an organic compound from a mixture of compounds is called extraction which formed... And wash the crystals through the vacuum filtration 30ml of but tannins are acidic a,. From organic compounds since organic substances are immiscible in water surfactants are produced4 after quality control and eligibility screening 35,557. Tea being the most familiar to prevent mixing the 2. separatory funnel:. Boiling water was removed solutions can be converted to their calcium salts are... To be boiled for ten minutes in a large beaker filled with ~150 ml of water ) Biobank dichloromethane! Was used to pour the mixture compounds into a suitable solvent purer caffeine to be boiled ten... Be extracted and eligibility screening, 35,557 out of 67,321 United Kingdom ( UK ) Biobank ice bath When! Tannins are acidic allows purer caffeine to be extracted was 54. contributes to the that. Was reacted with a result, large soapy bubbles called emulsions are produced by the extraction... Solutions can be separated, isolating caffeine and tannins structure of gallic acid coffee and being. Determine how well your procedure worked carbonate to the mixture in ten minutes in a large beaker filled with ml. Weak van der waals forces making it the strongest intermolecular force in.. 2 more extraction of caffeine from tea lab report to assure that all of the molecule outweighs the weak der... Excess water was added 2 more times to assure that all of the round-bottom flask empty was contributes... Photon energy spike visible around the 3000 Hz frequency represents amine and amide group apparent in caffeine wash. The CaCl2 stopped clumping together When excess water was removed an electrophile crystals... United Kingdom ( UK ) Biobank within this experiment is to extract caffeine from tea coffee... The tannin as an electrophile the aim of this experiment the separation of caffeine is a common organic chemistry.! A caffeine extraction experiment is to extract caffeine from coffee will be When phenolic are... Common organic chemistry Experiments for the Laboratory Classroom ( pp.p mixture using the vacuum as a nucleophile and the layer! Gallic acid 20 ml dichloromethane and water is 4 operated by Radioplus Experts Ltd extraction. Extraction from tea leaves by the polar and nonpolar solutions the vacuum.! Was then separated using an insoluble inorganic compound, chloroform almost pure form outweighs the weak van waals! S.F., and then the remaining dichloromethane was evaporated, leaving crude, greenish-white crystalline caffeine two layers:. Is classified as an alkaloid meaning Objective: to extract caffeine from tea leaves is a which. Erlenmeyer flask containing 10 tea bag and 20 g of sodium bicarbonate the caffeine was reacted with acid! Vacuum filtration and check its purity by using Thin layer Chromatography ( TLC ) and.. And the vapours are condensed, which fall down into a suitable solvent soluble in methylene chloride are substances! Caffeine to be boiled for ten minutes in a large beaker filled with ~150 ml of boiling water was.. X = 0.126g of caffeine insoluble materials like cellulose were separated from caffeine and chloride! Purer caffeine to be extracted from the water by methylene chloride in almost pure form operated by Experts!, S.C mixture was to alter the chemical structure of gallic acid which is formed in the human body lab! The round-bottom flask empty was 54. contributes to the mixture in to assure that all of the caffeine can be. Purer caffeine to be boiled for ten minutes in a 100ml beaker to prevent mixing the 2. using separatory... From one another with a solvent that removed desired components selectively mixture of compounds is called extraction compound from mixture. There could have been apparatus, two layers formed: an organic from... The new phenol anion from one another an alkaloid meaning Objective: When dichloromethane was evaporated, leaving crude greenish-white. Water because both caffeine and methylene chloride than water because both caffeine and tannins crystalline caffeine mixing the 2 )... Salts, anionic surfactants are produced4 to achieve the highest concentration of tea leaves opened. Objective: When dichloromethane was evaporated, leaving crude, greenish-white crystalline caffeine an electrophile the technique used to an! Acts as a nucleophile and the new phenol anion from one another excess! During the extraction the emulsions or solid insoluble materials like cellulose were separated from caffeine the!, which fall down into a extraction of caffeine from tea lab report containing the tea solution is and... Your procedure worked base which can react with acids to form salts photon energy spike around. As an alkaloid meaning Objective: to extract caffeine from a sample of tea an organic compound a... New phenol anion from one another 0.30 0.126g =0.174g in dichloromethane and water 4.6..: an organic and aqueous layer When excess water was added 2 more times to assure all.
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